نبذة مختصرة : The European hazelnut ( Corylus avellana L.) is a diploid (2n = 2x = 22), highly heterozygous tree nut crop. Eastern filbert blight (EFB) is caused by a fungal pathogen and is the most serious disease affecting hazelnut in North America. Breeding efforts have focused on developing molecular markers for identified EFB resistance sources, with the overarching goal of pyramiding multiple resistance alleles in elite selections.To this end, genomic resources representing distinct EFB resistance loci are being developed. These include haplotype-phased genome assemblies. The Oregon State University (OSU) breeding program has used molecular markers and linkage mapping to study EFB resistance, including the deployment of ‘Gasaway’, a dominant locus associated with EFB resistance in the PNW. EFB resistant cultivar ‘Jefferson,’ which carries ‘Gasaway’ resistance, has been developed as the reference genomefor Corylus . Here, we introduce a new, haplotype-phased chromosome-resolved genome assembly for ‘Jefferson,’ assembled using a trio-based approach. Both haplotype assemblies have N50 equal to ~32.5 Mbp, and approximately 30,800 predicted genes with a BUSCO score of ~90%. The haplotypes clearly differentiate candidate EFB resistance gene allelesinherited fromthe susceptible versus resistant parents. ; Presented at the 2023 Plant and Animal Genome conference in San Diego, USA.
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