نبذة مختصرة : Plant viral nanoparticles (VNPs) are attractive to nanomedicine researchers because of their safety, ease of production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits a high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain of staphylococcal Protein A via gene fusion. We selected cetuximab as a model IgG to demonstrate the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells from the Cal33 line of a tongue squamous cell carcinoma. Using confocal microscopy, we observed that fluorescent VNP–cetuximab bound selectively to Cal33 and was internalized, revealing the potential of this nanotool in cancer research. ; Ministerio de Universidades (España) ; Ministerio de Ciencia e Innovación (España) ; Comunidad de Madrid ; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) ; ERANet ; Universidad Complutense de Madrid ; Depto. de Genética, Fisiología y Microbiología ; Depto. de Biología Celular ; Fac. de Ciencias Biológicas ; Fac. de Medicina ; TRUE ; pub
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