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Antimicrobial Efficacy of XF-73 and Photo-Activated XF-73 Against Clinically Relevant Microorganisms in Planktonic and Biofilm Mode of Growth
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- معلومة اضافية
- الموضوع:
2022
- Collection:
Aston University: Aston Publications Explorer
- نبذة مختصرة :
Novel antimicrobials are urgently needed to combat the global increase in antimicrobial resistance. Exeporfinium chloride (XF-73) is a synthetic dicationic porphyrin antimicrobial, acting through membrane disruption; with a secondary mechanism of action, activated via blue light exposure. The aim of this study was to assess the antimicrobial efficacy of XF-73 and photo-activated XF-73 against a panel of clinically relevant Gram-positive and Gram-negative microorganisms in planktonic and biofilm modes of growth. The antimicrobial efficacy of XF-73 was assessed through minimum inhibitory and bactericidal concentration (MIC/MBC) assays. The biofilm Calgary device generated twenty-four hour biofilms to assess the anti-biofilm effect of XF-73 on biofilm viability through viable cell counts and through minimum biofilm inhibitory and eradication concentrations (MBIC/MBEC) assays. The antimicrobial activity of photo-activated XF-73 was also assessed following fifteen minutes blue light exposure at 420 nm (light dose = 13.8 J/cm2). XF-73 expressed a greater bactericidal activity against Gram-positive planktonic bacteria in comparison to Gram-negative microorganisms as MBCs ranged from ≤ 0.125 – 4 μg/ mL and 128 – >256 μg/ mL respectively. Photo-activation of XF-73 enhanced the bactericidal properties against both Gram-positive and Gram-negative bacteria as MBCs decreased to ≤ 0.03 – 1 μg/ mL and 32 – 128 μg/ mL. Gram-positive biofilms were highly susceptible to XF-73 with MBEC’s ranging from 1 – 2 μg/ mL. Photo-activation increased the bactericidal effect of XF-73 against Gram-positive biofilms with MBEC’s ranging between ≤ 0.125 – 0.5 μg/ mL. XF-73 was unable to eradicate Gram-negative biofilms, but concentrations 128 – 512 μg/ mL significantly reduced biofilm viability (p= <0.0001). XF-73 is a potent antimicrobial against Gram-positive microorganisms in both planktonic and biofilm modes of growth. Photo-activation further enhances the bactericidal effect of XF-73 and potentially offers an adjunct form of ...
- File Description:
text
- Relation:
https://publications.aston.ac.uk/id/eprint/45332/1/ROMEO_MELODY_ISABELLA_2022.pdf; Romeo-Melody, Isabella Liliana (2022). Antimicrobial Efficacy of XF-73 and Photo-Activated XF-73 Against Clinically Relevant Microorganisms in Planktonic and Biofilm Mode of Growth. PHD thesis, Aston University.
- Rights:
cc_by_nc_nd_4
- الرقم المعرف:
edsbas.42CB971
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