LBculture_wt

This is a LB culture of wt from a flask grown into stationary phase. The purpose was to to check that there are no nanowires and nothing was seen in sem, and when we do cryo-em to ensure we see flagellum. This would ensure that flagellum can't be seen in sem. But we did see long wires in sem. Sahand opined that is because we grew them into stationary phase. I imaged these to capture nanowires since they were present in sem ; Tilt Series Date: 2015-07-25 Data Taken By: Poorna Subramanian Species / Specimen: Shewanella oneidensis Strain: MR-1 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Moh and Sahand Purification / Growth Conditions / Treatment: Manual blotting Cu side glow discharged. Sample was put on opposite side and blotted. Similar to vitrobot ; Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2015-07-25-13 lbculture_nanowirepresent_atlas10012.mrc, Tilt Series (Pixel Size 0.48 nm), 1.7 GB Download lbculture_nanowirepresent_atlas10012_full.rec, Reconstruction (Pixel Size 1.92 nm), 445.4 MB Download