Additional file 4 of Preservation stress resistance of melanin deficient conidia from Paecilomyces variotii and Penicillium roqueforti mutants generated via CRISPR/Cas9 genome editing

Additional file 4: Figure S3. Diagnostic PCR on eight transformants in P. variotii PT39.26 missing the pvpP gene. a Diagnostic PCR to investigate the presence of the pvpP gene by amplifying outside the used flanks. If the gene is absent a band size of 2619 bps is expected. If the gene pvpP is still present, a band size of 9344 bps is present. The eight transformants all have lost the pvpP gene. The PCR fragments loaded on #6 and #7 were purified and subsequently send for sequencing. b Diagnostic PCR to investigate the presence of the pvpP gene by amplifying inside the gene. If the gene is absent, no band is expected. In wild-type situation, a PCR fragment of 1095 bps is expected. No transformants show the presence of pvpP gene. Taken together, these results show that 8/8 transformants had a full knock-out of the pvpP gene. Both contained the expected sequence for the repair DNA fragment, indicating repair by HDR.