نبذة مختصرة : Introduction: The tribes Cophomantini, Scinaxini and Dendropsophini are anurans that belong to Hylidae, with wide distribution in tropical and subtropical regions around the world. The taxonomy and systematics of this family remain in a state of ongoing revision. Previous cytogenetic analyses of genera Boana, Bokermannohyla, Ololygon, Scinax and Dendropsophus described some karyotypic characters such as conventional staining, C-banding and NORs, and FISH with specific probes. Methods: This study describes for the first time the karyotypes of four species Bokermannohyla ibitipoca, Ololygon luizotavioi, Dendropsophus bipunctatus and Dendropsophus ruschii. Furthermore, we map CA(15) and CAT(10) microsatellite sites and for the aforementioned species and six more species from the same genera, for the insight into the chromosomal evolution within subfamily Hyalinize. Results: Bok. ibitipoca and O. luizotavioi had 2n= 24 and karyotypic formulae 18m+4sm+2st and 8m+12sm+4st, while D. bipunctatus and D. ruschii showed 2n= 30 and karyotypic formulae 12m+12sm+4st+2t and 10m+10sm+6st+4t, respectively. The diploid numbers and karyotypic formulae revealed here follow the previously reported trend for Hylidae, except Bok. ibitipoca that has a particularity of eight metacentric chromosomes, more than what is commonly found in species of this genus. The microsatellites probes CA(15) and CAT(10) had markings accumulated in blocks in the centromeric, pericentromeric and terminal regions, that were more specific for some species, as well as markings scattered along the chromosomes. We present a comprehensive review table of current data on cytogenetics of these genera. Conclusion: Our findings showed that the karyotypes of the hylids studied here fit the postulated conserved diploid number and morphological chromosome patterns, while the mapping of these microsatellites enabled us to detect differences between species that share similar chromosomal morphologies.
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