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AtNPF5.5, a nitrate transporter affecting nitrogen accumulation in Arabidopsis embryo

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  • معلومة اضافية
    • Contributors:
      Biochimie et Physiologie Moléculaire des Plantes (BPMP); Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro); Institut National de la Recherche Agronomique (CJS PhD Fellowship) BAP2013-33-NITSE; Indo-French Centre for the Promotion of Advance Research (CEFIPRA) 4609-1; Agence Nationale de la Recherche ANR-11-JSV6-002-01-NUTSE; Agropolis Fondation (RHIZOPOLIS) 07024; Region Languedoc-Roussillon (Chercheur d'Avenir)
    • بيانات النشر:
      CCSD
      Nature Publishing Group
    • الموضوع:
      2014
    • Collection:
      Université de Montpellier: HAL
    • نبذة مختصرة :
      International audience ; Dipeptide (Leu-Leu) and nitrate transport activities of 26 Arabidopsis NPF (NRT1/PTR Family) proteins were screened in Saccharomyces cerevisiae and Xenopus laevis oocytes, respectively. Dipeptide transport activity has been confirmed for 2 already known dipeptide transporters (AtNPF8.1 and AtNPF8.3) but none of the other tested NPFs displays dipeptide transport. The nitrate transport screen resulted in the identification of two new nitrate transporters, AtNPF5.5 and AtNPF5.10. The localization of the mRNA coding for NPF5.5 demonstrates that it is the first NPF transporter reported to be expressed in Arabidopsis embryo. Two independent homozygous npf5.5 KO lines display reduced total nitrogen content in the embryo as compared to WT plants, demonstrating an effect of NPF5.5 function on the embryo nitrogen content. Finally, NPF5.5 gene produces two different transcripts (AtNPF5.5a and AtNPF5.5b) encoding proteins with different N-terminal ends. Both proteins are able to transport nitrate in xenopus oocytes.
    • ISBN:
      978-0-00-348164-8
      0-00-348164-6
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/25608465; PRODINRA: 306178; PUBMED: 25608465; PUBMEDCENTRAL: PMC4302306; WOS: 000348164600019
    • الرقم المعرف:
      10.1038/srep07962
    • الدخول الالكتروني :
      https://hal.science/hal-01141003
      https://hal.science/hal-01141003v1/document
      https://hal.science/hal-01141003v1/file/2015_L%C3%A9ran_Scientific%20Reports_1.pdf
      https://doi.org/10.1038/srep07962
    • Rights:
      info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.3AE0185