Additional file 1 of A novel mouse model for investigating α-synuclein aggregates in oligodendrocytes: implications for the glial cytoplasmic inclusions in multiple system atrophy ...

Additional file 1: Fig. S1. The αsynGFP in CNP-SNCAGFP Tg mice exhibits similar subcellular distribution to αsyn in CNP-SNCATg mice and does not aggregate with age. (A) Fluorescence micrographs of GFP and immunostaining with anti-human αsyn antibodies (red) and CNP (gray) of the striatum in CNP-SNCAGFP Tg mice and in CNP-SNCA Tg mice that express human αsyn in OLGs. Scale bar = 10 µm. (B) Western blot images of RIPA-soluble fraction with anti-αsyn and pαsyn antibodies in whole brains of Wt and Tg mice (2, 13, and 18 months old). The expression of endogenous mouse αsyn (arrow) and transgenic human αsynGFP fusion protein (arrowhead) are shown. (C) Latency to fall in the rotarod test at 12 months old. Student’s t-test was performed (not significant). Data indicate the mean ± SEM. Wt, wild-type; αsyn, alpha-synuclein; pαsyn, phosphorylated α-synuclein; PK, proteinase K; RT, room temperature. ...