نبذة مختصرة : Morphogenesis and cell-type differentiation are highly coordinated in sensory organs to ensure their function. Morphogenesis of the olfactory epithelium (OE) in zebrafish provides a unique model to study this process as undifferentiated cells aligned around the anterior neural plate mature into clusters of early olfactory neurons across a short time scale. While the Cxcl12a/Cxcr4b signaling pathway drives this process, what constraints on pathway activation apply during morphogenesis are unclear. We developed a mathematical model recapitulating Cxcl12a-mediated OE morphogenesis. Restoring Cxcl12a expression in mutants for the ligand rescues correct morphogenesis both in silico and in vivo. However, where expression of the ligand is restored is crucial for rescue, a point not predicted by our model and suggesting an unexpected level of pathway activation control. Analysis of a Cxcr4b activation reporter supports this idea. We concluded that mosaic and heterochronic Cxcl12a activation along the anteroposterior axis sculpts the olfactory epithelium.
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