Contributors: School of Geography, Earth and Environmental Sciences Birmingham; University of Birmingham Birmingham; Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro); Gestion de l'Eau, Acteurs, Usages (UMR G-EAU); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Bureau de Recherches Géologiques et Minières (BRGM)-Institut de Recherche pour le Développement (IRD)-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro; This work was supported by Institut Agro Montpellier, France and ALLEA MUSE project (from the French National Research Agency under the “Investissements d'avenir”, program number ANR-16-IDEX-0006). We thank EcoSec for providing the urine used in this experiment. We are grateful to the INRAE MIGALE bioinformatics facility for providing help and/or computing and/or storage resources.; ANR-16-IDEX-0006,MUSE,MUSE(2016)
نبذة مختصرة : Raw sequencing data has been deposited in NCBI's Sequence Read Archive (SRA) under the BioProject PRJNA1081346 (https://www.ncbi.nlm.nih.gov/sra/PRJNA1081346). Other datasets are available from the corresponding author on reasonable request. ; International audience ; Highlights: • After storage, urine microbiome was depleted but still had few common urine bacteria. • Urine fertilization did not affect the overall soil bacterial community structure. • Urine fertilization increased the abundance of nitrifying and denitrifying groups. • No bacterial salt stress was induced despite high urine salt concentration.Abstract: Using human urine as a crop fertilizer has sparked interest due to its potential benefits, but its application requires an understanding of how urine can affect soil functions and microbial communities. This study aims at elucidating the response of soil bacterial communities to fertilization with human urine. To this end, a spinach crop was fertilized with 2 different doses of a source-separated and stored human urine (170 kg N ha−1 + 8.5 kg P ha−1 and 510 kg N ha−1 + 25.5 kg P ha−1) and compared with a synthetic fertilizer treatment (170 kg N ha−1 + 8.5 kg P ha−1) and a water treatment without fertilization. The experiment was conducted in four soil tanks in greenhouse conditions, according to a randomized block scheme. We assessed urine and soil bacterial composition at the beginning and the end of the experiment that we compared to soil and plant properties to understand the drivers in bacterial composition changes. After 12 months of storage, urine had a depleted microbiome but still contained few common strains of urine or faeces. Overall, soil bacterial communities were resistant to urine fertilization with only 3 % of the taxa impacted. However, urine fertilization increased the relative abundance of nitrifying and denitrifying groups compared to the synthetic fertilizer implying that more N2O and NO could be emitted when fertilizing with urine. The urine's high salt concentration had ...
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