نبذة مختصرة : The aim of the study was isolation and characterization of species of Shigella genus in the cases of diarrhea in Abidjan. Shigella species were isolated from 1566 stool samples and characterized by serotyping, antibiotyping, determination of virulence genes and plasmid profile. A total of 41 (2.6% ) strains of Shigella were isolated. S. flexneri was the most frequent serogroup (52.8 %), followed by S. boydii (22.2%), S. sonnei (19.4%) and S. dysenteriae serotype 1 (5.6%). The ipaH gene was detected in 37 (90.2%) strains and the ial gene in 19 (46.3%). The strains show a high resistance to Ampicillin and Tetracyclin (90%), to Sulphamide (85%) and to a lesser extent to Cotrimoxazole (65% ). The analysis of the plasmid profile revealed 75% of the strains harbour plasmids whose size vary from 0.5 (0.8 kb) to 10.4 MDa (16.2 kb). The plasmids of size 1.7 MDa, 2.4 MDa and 3.6 Mda are frequent and are found in several species, whereas the plasmids of more than 10 MDa are rare. The analysis of the plasmid profile and the detection of the virulence factors can serve as tools in the characterization of strains and the control of infections associated with Shigella.
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