نبذة مختصرة : The strictosidine synthase-like ( SSL ) gene family is a small plant immune-regulated gene family that plays a critical role in plant resistance to biotic/abiotic stresses. To date, very little has been reported on the SSL gene in plants. In this study, a total of thirteen SSL s genes were identified from poplar, and these were classified into four subgroups based on multiple sequence alignment and phylogenetic tree analysis, and members of the same subgroup were found to have similar gene structures and motifs. The results of the collinearity analysis showed that poplar SSL s had more collinear genes in the woody plants Salix purpurea and Eucalyptus grandis . The promoter analysis revealed that the promoter region of PtrSSL s contains a large number of biotic/abiotic stress response elements. Subsequently, we examined the expression patterns of PtrSSL s following drought, salt, and leaf blight stress, using RT-qPCR to validate the response of PtrSSL s to biotic/abiotic stresses. In addition, the prediction of transcription factor (TF) regulatory networks identified several TFs, such as ATMYB46 , ATMYB15 , AGL20 , STOP1 , ATWRKY65 , and so on, that may be induced in the expression of PtrSSL s in response to adversity stress. In conclusion, this study provides a solid basis for a functional analysis of the SSL gene family in response to biotic/abiotic stresses in poplar.
Processing Request
No Comments.