Contributors: Béchir Hamza Children's Hospital; Laboratoire de Bioinformatique, biomathématiques, biostatistiques (BIMS) (LR11IPT09); Université de Tunis El Manar (UTM)-Institut Pasteur de Tunis; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP); Université de Tunis El Manar (UTM); Hôpital Charles Nicolle Tunis; Laboratoire de Virologie Clinique, Référence Régional OMS pour la Poliomyélite et la Rougeole - Laboratory of Clinical Virology, WHO Regional Reference Laboratory on Poliomyelitis and Measles; Institut Pasteur de Tunis; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Organisation Mondiale de la Santé / World Health Organization Office Genève, Suisse (OMS / WHO); Virus, Vecteurs et Hôtes Tunis; This work was supported by the Research and High Education Ministry of Tunisia Research Laboratory LR18ES39 .; The authors are grateful to Pr Amel Kechrid, Pr Nejla Ben Jeballah and the staff of the Pediatric Intensive Care Unit (PICU) of Bechir Hamza Children’s Hospital in Tunis, Tunisia, for their help in collecting samples and patient’s information.
نبذة مختصرة : International audience ; Rhinoviruses (RV) are a major cause of Severe Acute Respiratory Infection (SARI) in children, with high genotypic diversity in different regions. However, RV type diversity remains unknown in several regions of the world. In this study, the genetic variability of the frequently circulating RV types in Northern Tunisia was investigated, using phylogenetic and phylogeographic analyses with a specific focus on the most frequent RV types: RV-A101 and RV-C45. This study concerned 13 RV types frequently circulating in Northern Tunisia. They were obtained from respiratory samples collected in 271 pediatric SARI cases, between September 2015 and November 2017. A total of 37 RV VP4-VP2 sequences, selected among a total of 49 generated sequences, was compared to 359 sequences from different regions of the world. Evolutionary analysis of RV-A101 and RV-C45 showed high genetic relationship between different Tunisian strains and Malaysian strains. RV-A101 and C45 progenitor viruses' dates were estimated in 1981 and 1995, respectively. Since the early 2000s, the two types had a wide spread throughout the world. Phylogenetic analyses of other frequently circulating strains showed significant homology of Tunisian strains from the same epidemic period, in contrast with earlier strains. The genetic relatedness of RV-A101 and RV-C45 might result from an introduction of viruses from different clades followed by local dissemination rather than a local persistence of an endemic clades along seasons. International traffic may play a key role in the spread of RV-A101, RV-C45, and other RVs.
Processing Request
No Comments.