Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Purification and Kinetic Studies of Lipases Using Reversed Micellar Systems

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Contributors:
      Laboratério de Engenharia Bioquimica, Instituto Superior Técnico, 1000 Lisboa, Portugal
    • بيانات النشر:
      GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
    • الموضوع:
      2024
    • Collection:
      Helmholtz Centre of Infection Research (HZI), Braunschweig: Repository
    • نبذة مختصرة :
      Selective separation and purification of two lipases (A and B) from Chromobacterium viscosum were carried out by liquid-liquid extraction using a reversed micellar system (250 mM AOTin isooctane), with selective extraction of lipase B to the micellar phase and lipase A remaining in aqueous solution. The purified lipases were used to catalyze esterification reactions between carboxylic acids and alcohols in reversed micelles. The influence of various parameters in the synthesis of oleyl propionate by lipases A and B, was studied. The maximum activity was obtained at Wo([H20]/[AOT])=6.7.
    • ISBN:
      978-1-56081-165-7
      978-3-527-28332-3
      1-56081-165-X
      3-527-28332-3
    • ISSN:
      0930-4320
    • Relation:
      GBF monographs; Volume 16; Lipases : structure, mechanism and genetic engineering, 407 - 416; 156081165X; http://hdl.handle.net/10033/623750; Lipases : structure, mechanism and genetic engineering, 1991
    • Rights:
      Attribution-NonCommercial-ShareAlike 4.0 International ; http://creativecommons.org/licenses/by-nc-sa/4.0/
    • الرقم المعرف:
      edsbas.328C8771