Assessment of genome assembly and annotation completeness using single-copy ortholog benchmarking.

5 PacBio-assembled genomes with annotation were compared using either ‘eukaryota_odb9’ or ‘protists_ensembl’ datasets using default parameters. The ‘eukaryota_odb9’ dataset contains 303 single-copy genes conserved in 100 eukaryote species, while the “protists_ensembl’ dataset’ contains 215 single-copy genes that are present among 33 protist species. *Note that TCC is a hybrid strain, so its genome is a mixture of two haplotypes, while all other genomes contain one haplotype. (PDF)