THE EFFECT OF MOLECULAR HYDROGEN ON OXIDATIVE MODIFICATION OF PROTEINS IN COLORECTAL CANCER IN THE EXPERIMENT ; ВПЛИВ МОЛЕКУЛЯРНОГО ВОДНЮ НА ОКИСНЮВАЛЬНУ МОДИФІКАЦІЮ ПРОТЕЇНІВ ПРИ КОЛОРЕКТАЛЬНОМУ РАКУ В ЕКСПЕРИМЕНТІ

Introduction. An important role in the pathogenesis of the development of colorectal cancer is played by oxidative stress or imbalance of prooxidant/antioxidant homeostasis, which leads to oxidative modification of proteins and enhanced formation of protein carbonyl groups. The presence of oxidative stress in colorectal cancer requires effective antioxidant therapy. In recent years, there has been a growing interest in the study of molecular hydrogen as an inert gas that effectively exhibits an antioxidant effect. The aim of the study – to investigate the effect of water saturated with molecular hydrogen on the content of carbonyl derivatives of oxidatively modified proteins in the blood serum of white rats with colorectal cancer. Research Methods. Experiments were conducted on 50 male Wistar white rats. Colorectal cancer (CRC) was simulated in animals by subcutaneous injection of 1,2-dimethylhydrazine at a dose of 7.2 mg/kg of body weight once a week for 30 weeks. Rats consumed water saturated with molecular hydrogen at a concentration of 0.6 ppm ad libitum. Animals were euthanized under thiopental anesthesia. Blood serum was used for the study, in which the content of carbonyl groups was determined by the colorimetric method. Statistical data processing was performed using the SPSS22 software package. Results and Discussion. It was established that the modeling of colorectal cancer led to an increase in the content of carbonyl groups in the blood serum of rats by 1.93 times compared to intact animals. The content of carbonyl groups in the blood serum of rats with colorectal cancer that consumed water saturated with molecular hydrogen for 30 weeks in parallel with the administration of 1,2-dimethylhydrazine was 1.29 times lower than in animals with colorectal cancer that consumed ordinary water . Consumption of water saturated with molecular hydrogen for 30 days after modeling colorectal cancer in white rats also led to a decrease in the content of carbonyl groups in their blood serum. Conclusions. The use of ...