Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Interaction of the H-cluster of FeFe hydrogenase with halides

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • معلومة اضافية
    • Contributors:
      Bioénergétique et Ingénierie des Protéines (BIP ); Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS); Dipartimento di Biotecnologie e Bioscienze; Università degli Studi di Milano-Bicocca = University of Milano-Bicocca (UNIMIB); Department of Biotechnology and Biosciences; ANR-12-BS08-0014,ECCHYMOSE,Etudes d'hydrogénases à Fer par électrochimie: mécanisme et optimisation pour la photoproduction d'hydrogène(2012); ANR-14-CE05-0010,HEROS,Hydrogénases résistantes à l'Oxygène(2014); ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011)
    • بيانات النشر:
      HAL CCSD
      American Chemical Society
    • الموضوع:
      2018
    • Collection:
      Aix-Marseille Université: HAL
    • نبذة مختصرة :
      International audience ; FeFe hydrogenases catalyse H2 oxidation and production using a "H-cluster", where two Fe ions are bound by an aza-dithiolate (adt) ligand. Various hypotheses have been proposed (by us and others) to explain that the enzyme reversibly inactivates under oxidizing, anaerobic conditions: intramolecular binding of the N atom of adt, formation of the so-called Hox/inact state or non-productive binding of H2 to isomers of the H-cluster. Here we show that none of the above explains the new finding that the anaerobic, oxidative, H2-dependent reversible inactivation is strictly dependent on the presence of Cl- or Br-. We provide experimental evidence that chloride uncompetitively inhibits the enzyme: it reversibly binds to catalytic intermediates of H2 oxidation (but not to the resting "Hox" state), after which oxidation locks the active site into a stable, saturated, inactive form, the structure of which is proposed here based on DFT calculations. The halides interact with the amine group of the H-cluster but do not directly bind to iron. It should be possible to stabilize the inhibited state in amounts compatible with spectroscopic investigations to explore further this unexpected reactivity of the H-cluster of hydrogenase.
    • Relation:
      hal-01759718; https://amu.hal.science/hal-01759718; https://amu.hal.science/hal-01759718/document; https://amu.hal.science/hal-01759718/file/halides%20author%20version%20HAL%201759718.pdf
    • الرقم المعرف:
      10.1021/jacs.8b01414
    • الدخول الالكتروني :
      https://amu.hal.science/hal-01759718
      https://amu.hal.science/hal-01759718/document
      https://amu.hal.science/hal-01759718/file/halides%20author%20version%20HAL%201759718.pdf
      https://doi.org/10.1021/jacs.8b01414
    • Rights:
      info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.2AA8F4C5