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3D-printed platform multi-loaded with bioactive, magnetic nanoparticles and an antibiotic for re-growing bone tissue

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • بيانات النشر:
      Elsevier
    • الموضوع:
      2024
    • Collection:
      Universidade de Lisboa: repositório.UL
    • نبذة مختصرة :
      Polymeric platforms obtained by three-dimensional (3D) printing are becoming increasingly important as multifunctional therapeutic systems for bone treatment applications. In particularly, researchers aim to control bacterial biofilm on these 3D-platforms and enhance re-growing bone tissue, at the same time. This study aimed to fabricate a 3D-printed polylactic acid platform loaded with hydroxyapatite (HA), iron oxide nanoparticles (IONPs) and an antibiotic (minocycline) with tuneable properties and multistimuli response. IONPs were produced by a facile chemical co-precipitation method showing an average diameter between 11 and 15 nm and a superparamagnetic behaviour which was preserved when loaded into the 3D-platforms. The presence of two types of nanoparticles (IONPs and HA) modify the nanomorphological/nanotopographical feature of the 3D-platforms justifying their adequate bioactivity profile and in vitro cellular effects on immortalized and primary osteoblasts, including cytocompatibility and increased osteogenesis-related gene expression (RUNX2, BGLAP and SPP1). Disk diffusion assays and SEM analysis confirmed the effect of the 3D-platforms loaded with minocycline against Staphylococcus aureus. Altogether results showed that fabricated 3D-platforms combined the exact therapeutic antibiofilm dose of the antibiotic against S. aureus, with the enhanced osteogenic stimulation of the HA and IONPs nanoparticles which is a disruptive approach for bone targeting applications. ; Support for this work was provided by National Funds through FCT - Fundação para a Ciência e Tecnologia, I.P, under the projects with references UIDB/04138/2020 and UIDP/04138/2020, PTDC/BTM-SAL/29335/2017, UIDB/50022/2020, CQE-UIDB/00100/2020, UIDB/50006/2020, UID/Multi/04349/2013, PDTC/QUI-QIN/32240/2017 and GCT grant to A.C.C. (BL156/2019_IST-ID). ; info:eu-repo/semantics/acceptedVersion
    • ISSN:
      0378-5173
    • Relation:
      info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT; info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F29335%2F2017/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50022%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04349%2F2013/PT; PDTC/QUI-QIN/32240/2017; https://www.sciencedirect.com/science/article/pii/S0378517320310826; Saraiva AS, Ribeiro IAC, Fernandes MH, Cerdeira AC, Vieira BJC, Waerenborgh JC, et al. 3D-printed platform multi-loaded with bioactive, magnetic nanoparticles and an antibiotic for re-growing bone tissue. International Journal of Pharmaceutics [Internet]. janeiro de 2021;593:120097. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0378517320310826; http://hdl.handle.net/10451/62047; cv-prod-2118917
    • الرقم المعرف:
      10.1016/j.ijpharm.2020.120097
    • الدخول الالكتروني :
      https://doi.org/10.1016/j.ijpharm.2020.120097
      http://hdl.handle.net/10451/62047
    • Rights:
      openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/
    • الرقم المعرف:
      edsbas.2660E96C