Epigallocatechin-3-gallate (EGCG): an alternative to extenuate occupational risk factors outcomes? An interventional study

FCT_UIDB/05608/2020. FCT_UIDP/05608/2020. ; Introduction: Occupational risk factors are major players in the increased risk of cardiovascular diseases and cancer. Studies support a protective role of Epigallocatechin-3-gallate (EGCG) in disease onset, with associated antioxidant properties and reactive oxygen species production. We aimed to evaluate the in vivo effects of EGCG intake on cardiovascular risk factors, DNA damage, and oxidative DNA damage. Methods: Voluntaries were enrolled in this interventional study with the safeguard of all ethical considerations. Peripheral blood was collected at the beginning and after 90 days of 225 mg EGCG ingestion per day. Lipid profile and liver function parameters were assessed using colorimetric methods. Vitamins A and E in serum were quantified by HPLC–DAD. DNA damage and oxidative DNA damage were assessed through comet assay. Results: Vitamin A, as well as the lipid profile and liver function parameters, were not affected by EGCG intake, whereas serum levels of vitamin E, DNA damage, and DNA oxidative damage increased after EGCG consumption. Discussion/Conclusions: EGCG induces low-level oxidative stress which may trigger protective antioxidant systems associated with vitamin E. Further research is crucial to understand the extent of EGCG effects and its potential as an alternative to extenuate occupational risk factors outcomes. ; H&TRC authors gratefully acknowledge the FCT/MCTES national support and the financial and institutional support of the Health & Technology Research Center, ESTeSL- Escola Superior de Tecnologia da Saúde, Instituto Politécnico de Lisboa. ; info:eu-repo/semantics/publishedVersion