Never change a flowing system? The effects of retrograde flow on isolated perfused lungs and vessels

Retrograde perfusion may occur during disease, surgery or extracorporeal circulation. While it is clear that endothelial cells sense and respond to changes in blood flow, the consequencesof retrograde perfusion are only poorly defined. Similar to shear stress or disturbed flow, retrogradeperfusion might result in vasomotor responses, edema formation or inflammation in and around vessels. In this study we investigated in rats the effects of retrograde perfusion in isolated systemicvessels (IPV) and in pulmonary vessels of isolated perfused lungs (IPL). Anterograde and retrogradeperfusion was performed for 480 min in IPV and for 180 min in the IPL. Perfusion pressure, cytokinelevels in perfusate and bronchoalveolar lavage fluid (BALF), edema formation and mRNA expressionwere studied. In IPV, an increased perfusion pressure and initially also increased cytokine levelswere observed during retrograde perfusion. In the IPL, increased edema formation occurred, while cytokine levels were not increased, though dilution of cytokines in BALF due to pulmonary edemacannot be excluded. In conclusion, effects of flow reversal were visible immediately after initiation ofretrograde perfusion. Pulmonary edema formation was the only effect of the 3 h retrograde perfusion. Therefore, further research should focus on identification of possible long-term complications of flow reversal.