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Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA

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  • معلومة اضافية
    • بيانات النشر:
      Nature Publishing Group
    • الموضوع:
      2015
    • Collection:
      The University of Adelaide: Digital Library
    • نبذة مختصرة :
      Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement reaction rate without altering other reaction parameters and provides new insights into the influence of single nucleotide substitutions on 3- and 4-way branch migration efficiency and kinetics. ; Dmitriy A. Khodakov, Anastasia S. Khodakova, David M. Huang, Adrian Linacre, Amanda V. Ellis
    • File Description:
      application/pdf
    • ISSN:
      2045-2322
    • Relation:
      http://purl.org/au-research/grants/arc/FT130100211; Scientific Reports, 2015; 5(1):8721-1-8721-8; http://hdl.handle.net/2440/91359; Huang, D. [0000-0003-2048-4500]
    • الرقم المعرف:
      10.1038/srep08721
    • Rights:
      This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
    • الرقم المعرف:
      edsbas.15B9251F