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Genome sequencing of two Bell pepper endornavirus (BPEV) variants infecting Capsicum annuum in Colombia ; Secuenciación del genoma de dos variantes de Bell pepper endornavirus (BPEV) que infectan Capsicum annuum en Colombia

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  • معلومة اضافية
    • بيانات النشر:
      Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
    • الموضوع:
      2017
    • Collection:
      Universidad Nacional de Colombia: Portal de Revistas UN
    • نبذة مختصرة :
      Transcriptome analysis of chili and bell pepper samples from commercial plots in the municipalities of Santa Fe de Antioquia and El Peñol in the province of Antioquia revealed the presence of viral sequences with significant similarity to genomes of members of the genus Endornavirus. Assembly of the chili and bell pepper transcriptomes resulted in consensus sequences of 14,727 nt and 14,714 nt that were identified as Bell pepper endornavirus (BPEV). Both sequences were nearly identical by 99.9 % at both nucleotide and amino acid levels. The presence of BPEV was confirmed by RT-qPCR, RT-PCR and Sanger sequencing using RdRp-specific primers designed from the assembled sequences in ten independent random samples taken from the investigated bell pepper stands. The phylogenetic analysis of both BPEV variants and their affiliation within the genus Endornavirus is discussed. For our knowledge, this is the first study on this group of viruses in Colombia. ; El análisis de transcriptomas de ají y pimentón en muestras obtenidas de cultivos comerciales en Santa Fe de Antioquia y El Peñol en el departamento de Antioquia, reveló la presencia de secuencias virales con similitud significativa con miembros del género Endornavirus. El ensamblaje de los transcriptomas arrojó secuencias consenso de 14.727 nt y 14.714 nt que fueron identificadas como los genomas de la especie viral Bell pepper endornavirus(BPEV), al compartir con ésta un 99,9% de identidad a nivel de nucleótidos y aminoácidos. La presencia de BPEV fue confirmada en diez muestras foliares de pimentón mediante pruebas de RT-qPCR, RT-PCR y secuenciación parcial de la región RdRp con cebadores específicos diseñados en este trabajo. La relación filogenética de ambas variantes de BPEV con otros miembros del género Endornavirus es analizada y discutida. Para nuestro conocimiento, este es el primer trabajo reportado sobre este grupo de virus en Colombia.
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    • Relation:
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    • الدخول الالكتروني :
      https://revistas.unal.edu.co/index.php/agrocol/article/view/60626
    • Rights:
      Derechos de autor 2017 Agronomía Colombiana ; https://creativecommons.org/licenses/by-nc-sa/4.0
    • الرقم المعرف:
      edsbas.13D48460