Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

PTPN13 Participates in the Regulation of Epithelial–Mesenchymal Transition and Platinum Sensitivity in High-Grade Serous Ovarian Carcinoma Cells

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • معلومة اضافية
    • Contributors:
      Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM); CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM); Institut National de la Santé et de la Recherche Médicale (INSERM); Institut régional de Cancérologie de Montpellier (ICM); Centre National de la Recherche Scientifique (CNRS); This research was funded by the “Ligue Nationale Contre le Cancer (comité du Gard), grant number R20025FF FREISS”.
    • بيانات النشر:
      CCSD
      MDPI
    • الموضوع:
      2023
    • Collection:
      Université de Montpellier: HAL
    • نبذة مختصرة :
      International audience ; Epithelial ovarian cancer (EOC) is the leading cause of death from gynecological cancers in Western countries. High-Grade Serous Ovarian Carcinoma (HGSOC) accounts for 60–70% of EOC and is the most aggressive subtype. Reduced PTPN13 expression levels have been previously correlated with worse prognosis in HGSOC. However, PTPN13’s exact role and mechanism of action in these tumors remained to be investigated. To elucidate PTPN13’s role in HGSOC aggressiveness, we used isogenic PTPN13-overexpressing clones of the OVCAR-8 cell line, which poorly expresses PTPN13, and also PTPN13 CRISPR/Cas9-mediated knockout/knockdown clones of the KURAMOCHI cell line, which strongly expresses PTPN13. We investigated their migratory and invasive capacity using a wound healing assay, their mesenchymal-epithelial transition (EMT) status using microscopy and RT-qPCR, and their sensitivity to chemotherapeutic drugs used for HGSOC. We found that (i) PTPN13 knockout/knockdown increased migration and invasion in KURAMOCHI cells that also displayed a more mesenchymal phenotype and increased expression of the SLUG, SNAIL, ZEB-1, and ZEB-2 EMT master genes; and (ii) PTPN13 expression increased the platinum sensitivity of HGSOC cells. These results suggest that PTPN13 might be a predictive marker of response to platinum salts in HGSOC.
    • الرقم المعرف:
      10.3390/ijms242015413
    • الدخول الالكتروني :
      https://hal.science/hal-04285044
      https://hal.science/hal-04285044v1/document
      https://hal.science/hal-04285044v1/file/Aptecar%202023%20PTPN13%20EMT%20platinum%20salts%20HGSOC.pdf
      https://doi.org/10.3390/ijms242015413
    • Rights:
      info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.11AAEA99