نبذة مختصرة : Carboxysomes purified from H. Neapolitanus, WT, high resolution ; Tilt Series Date: 2019-08-21 Data Taken By: LaurenAnn Metskas Species / Specimen: Halothiobacillus neapolitanus Strain: c2 Tilt Series Settings: Single Axis, tilt range: (-66.0°, 66.0°), step: 3°, constant angular increment, dosage: 120.0 eV/Ų, defocus: 0.0 μm, magnification: 81000x. Acquisition Software: Serial EM Upload Method: rundir Processing Software Used: IMOD, ctffind4, novaCTF, custom bash and Matlab scripts Collaborators and Roles: purified by Tom Laughlin in the Savage lab at Berkeley; data collected by Lauren Ann Metskas; data processed by Lauren Ann Metskas Purification / Growth Conditions / Treatment: purified by the Savage lab Sample Preparation: protochip C-flat 2/2-300 grid, 10nm BSA-gold, 100% humidity, defocus varying -1 to -5.5 microns ; Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/lma2019-08-21-25 25_dose-filt.st, Tilt Series (Pixel Size 0.1104 nm), 4.1 GB Download 25_dose-filt.rec, Reconstruction (Pixel Size 0.8832 nm), 479.2 MB Download scripts.tar.bz2, zipped processing scripts, 12.9 kB Download frames.tar.bz2, zipped raw frames, 1.7 GB Download TS_25.mrc.mdoc, , 30.2 kB Download
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