Modulating lipid membrane morphology by dynamic DNA origami networks

Membrane morphology and its dynamic adaptation regulate many cellular functions, which are often mediated by membrane proteins. Advances in DNA nanotechnology have enabled the realization of various protein-inspired structures and functions with precise control at the nanometer level, suggesting a viable tool to artificially engineer the membrane morphology. In this work, we demonstrate a DNA origami cross (DOC) structure that can be anchored onto giant unilamellar vesicles (GUVs) and subsequently polymerized into micron-scale reconfigurable one-dimensional (1D) chains or two-dimensional (2D) lattices. Such DNA origami-based networks can be switched between left-handed (LH) and right-handed (RH) conformations by DNA fuels and exhibit potent efficacy in remodeling the membrane curvatures of GUVs. This work sheds light on designing hierarchically-assembled dynamic DNA systems for the programmable modulation of synthetic cells for useful applications.
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano Letters, copyright American Chemical Society after peer review. To access the final edited and published work see Nano Lett. 2023, 23, 14, 6330-6336