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Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity

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  • معلومة اضافية
    • Contributors:
      Hémostase, Inflammation, Thrombose (HITH - U1176 Inserm - CHU Bicêtre); Université Paris-Sud - Paris 11 (UP11)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre)-Institut National de la Santé et de la Recherche Médicale (INSERM); Vascular research center of Marseille (VRCM); Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU); Service d'hématologie, immunologie biologiques et cytogénétique; Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre; Institut de Recherches Internationales Servier [Suresnes] (IRIS); The study was financially supported by a PhD-research grant from Institut Servier & Association Nationale de la Recherche Technique (Contrat CIFRE) to JPe, and an Inserm-Poste d’Accueil-grant (# ASC13101LSA) to MP.; Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre); Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
    • الموضوع:
      2016
    • نبذة مختصرة :
      International audience; Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. Pre-treatment of PSGL1 with sialidase reduced Siglec-5 binding by 79 ± 4%. In confocal immune-fluorescence assays, we observed that 50% of Peripheral Blood Mononuclear Cells (PBMCs) simultaneously express PSGL1 and Siglec-5. Duolink-proximity ligation analysis demonstrated that PSGL1 and Siglec-5 are in close proximity (
    • ISSN:
      2045-2322
    • الرقم المعرف:
      10.1038/srep37953⟩
    • Rights:
      OPEN
    • الرقم المعرف:
      edsair.pmid.dedup....b0ddd5764163b44fce0bc2bdd8f26d60