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Detection of SARS-CoV-2 spike protein binding to ACE2 in living cells by TR-FRET

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  • معلومة اضافية
    • Contributors:
      Institut Cochin (IC UM3 (UMR 8104 / U1016)); Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP); ANR-20-COV4-0001,MELATOVID,Mélatonine et médicaments mélatoninergiques pour la prévention et le traitement de COVID-19(2020); ANR-19-CE16-0025,mitoGPCR,Les récepteurs couplés aux protéines G mitochondriaux en neuroprotection(2019); ANR-16-CE18-0013,Tbeta-ORPH,Nouvelles fonctions pour les récepteurs orphelins couplés aux protéines G – formation d'un complexe entre le récepteur TGFß et GPR50(2016)
    • بيانات النشر:
      Elsevier BV, 2022.
    • الموضوع:
      2022
    • نبذة مختصرة :
      The SARS-CoV-2 coronavirus infects human cells through the interaction of the viral envelope spike protein (IPR044366) with the human angiotensin-converting enzyme 2 (ACE2), expressed at the surface of target cells. Here, we describe a detailed protocol to measure the binding of the receptor binding domain (RBD) of spike to ACE2 by time-resolved fluorescence resonance energy transfer (TR-FRET). The assay detects the spike/ACE2 interaction in physiologically relevant cellular contexts and is suitable for high-throughput investigation of interfering small-molecule compounds and antibodies. For complete details on the use and execution of this protocol, please refer to Cecon et al. (2021).
    • ISSN:
      2666-1667
    • الرقم المعرف:
      10.1016/j.xpro.2021.101024
    • Rights:
      CC BY NC ND
      URL: http://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
    • الرقم المعرف:
      edsair.doi.dedup.....fb0974025eb75daf25214f6840b5c513