Role of Altered Arachidonic Acid Metabolism in 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Immune Suppression in C57Bl/6 Mice

Role of Altered Arachidonic Acid Metabolism in 2,3,7,8-Tetrachlo- rodibenzo-p-dioxin-Induced Immune Suppression in C57B1/6 Mice. Lawrence, B. P., and Kerkvliet, N. I. (1998). Toxicol. Sci. 42, 13-22. One of the most sensitive targets of 23,7,8-tetrac hlorodibenzo-p- dioxin (TCDD) is the immune system. Many arachidonic acid (AA) metabolites are potent immunoregulatory molecules, and in other systems, TCDD has been shown to alter AA metabolism. Further- more, the genes for cyclooxygenase (cox) contain a dioxin response element, suggesting that exposure to TCDD may directly alter cox levels and prostaglandin (PG)^ production. To test the hypothesis that TCDD induces immune suppression by altering the production of immunomodulat ory AA metabolites, we examined the effects of TCDD on splenic AA release, LTB4 and PGEj production, and cox-1 and cox-2 expression. Exposure of C57B1/6 mice to TCDD (15 (xg/kg) resulted in a 2-fold increase in the release of AA from spleen cell membranes, a 1.4-fold enhancement of LTB4 and PGEj production in the spleen, and 3-fold higher PGEj levels in the peritoneal cavity during the immune response to allogeneic P815 tumor cells. We examined the direct induction of cox-1 and cox-2 by TCDD and the indirect induction of cox-2 via TCDD-induced IL-1. Interestingly, exposure to TCDD did not alter message or protein levels of cox-1, cox-2, or IL-1 over the course of the response to P815. Various metabolic inhibitors were then used to address the in vivo role of TCDD-induced changes in AA metabolism. While these inhibitors blocked AA metabolism, they failed to affect the TCDD-induced suppression of either the cytotoxic T lymphocyte response to P815 tumor cells or antibody formation in response to sheep red blood cells. The lack of effect of TCDD on cox expression, combined with the failure of metabolic inhibitors to reverse the suppression caused by TCDD, supports the conclusion that TCDD immunotoxicity is likely not mediated by a direct effect on the production of immunomodu- latory AA metabolites, o iws scday