Salivary Glands Proteins Expression ofAnopheles dirusA Fed onPlasmodium vivax-andPlasmodium falciparum-Infected Human Blood

Mosquitoes are able to adapt to feed on blood by the salivary glands which created a protein that works against the haemostasis process. This study aims to investigate the salivary glands proteins expression of 50 adult femaleAn. dirusA mosquitoes, a main vector of malaria in Thailand, each group with an age of 5 days which were artificial membrane fed on sugar, normal blood, blood infected withP. vivax, and blood infected withP. falciparum. Then mosquito salivary gland proteins were analyzed by SDS-PAGE on days 0, 1, 2, 3, and 4 after feeding. The findings revealed that the major salivary glands proteins had molecular weights of 62, 58, 43, 36, 33, 30, and 18 kDa. One protein band of approximately 13 kDa was found in normal blood and blood infected withP. vivaxfed on day 0. A stronger protein band, 65 kDa, was expressed from the salivary glands of mosquitoes fed withP. vivax- orP. falciparum-infected blood on only day 0, but none on days 1 to 4. The study shows that salivary glands proteins expression ofAn. dirusmay affect the malaria parasite life cycle and the ability of mosquitoes to transmit malaria parasites in post-24-hour disappearance observation.