نبذة مختصرة : Repair of broken DNA by homologous recombination requires coordinated enzymatic reactions to prepare it for interaction with intact DNA. The multiple activities of enterobacterial RecBCD helicase-nuclease are coordinated by Chi recombination hotspots (5’ GCTGGTGG 3’) recognized during DNA unwinding. Chi is recognized in a tunnel in RecC but activates the RecB nuclease, >25 Ǻ away. How the Chi-dependent signal travels this long distance has been unknown. We found a Chi-recognition-deficient mutant in the RecB helicase domain located >45 Ǻ from both the Chi-recognition site and the nuclease active site. This unexpected observation led us to find additional mutations that reduced or eliminated Chi hotspot activity in each subunit and widely scattered throughout RecBCD. Each mutation alters the intimate contact between one or another pair of subunits in the crystal or cryoEM structures of RecBCD bound to DNA. Collectively, these mutations span a path ∼185 Ǻ long from the Chi recognition site to the nuclease active site. We discuss these surprising results in the context of an intramolecular signal transduction accounting for many previous observations.
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