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Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis

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  • معلومة اضافية
    • Contributors:
      Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB); Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier); Pathogénèse et contrôle des infections chroniques (PCCI); Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ); Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier); CIC Montpellier; Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-CHU Saint-Eloi-Institut National de la Santé et de la Recherche Médicale (INSERM); Firalis SAS; Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM); CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)
    • بيانات النشر:
      MDPI AG, 2019.
    • الموضوع:
      2019
    • نبذة مختصرة :
      Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory rheumatism in childhood
      microRNAs (miRNAs) have been proposed as diagnostic biomarkers. Although joints are the primary targets for JIA, a synovial fluid-based miRNA signature has never been studied. We aim to identify miRNA biomarkers in JIA by comparing synovial fluid and serum samples from children with JIA and K. kingae septic arthritis (SA). With next-generation high-throughput sequencing, we measured the absolute levels of 2083 miRNAs in synovial fluid and serum from an exploratory cohort of children and validated differentially expressed miRNAs in a replication study by using RT-qPCR. We identified a 19-miRNA signature only in synovial fluid samples that was significantly deregulated, with at least 2-fold change in expression, in JIA versus SA (p <
      0.01). The combination of miR-6764-5p, miR-155, and miR-146a-5p expression in synovial fluid yielded an area under the receiver operating characteristic curve of 1 (95% CI 0.978 to 1), thereby perfectly differentiating JIA from SA in children. We propose, for the first time, a synovial fluid-specific miRNA signature for JIA and associated signaling pathways that may indicate potential biomarkers to assist in the classification and differential diagnosis of JIA and help in understanding JIA pathogenesis.
    • File Description:
      application/pdf
    • ISSN:
      2073-4409
    • الرقم المعرف:
      10.3390/cells8121521
    • الرقم المعرف:
      10.3390/cells8121521⟩
    • Rights:
      OPEN
    • الرقم المعرف:
      edsair.doi.dedup.....c2099912436618329ca00c20fbe08800