Immobilization of a Plant Lipase from Pachira aquatica in Alginate and Alginate/PVA Beads

Made available in DSpace on 2015-04-27T11:55:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2014Bitstream added on 2015-04-27T12:08:31Z : No. of bitstreams: 1 ISSN2090-0406-2014-2014-01-07.pdf: 1814271 bytes, checksum: 3e50b07227c28d70c31556fc9460436c (MD5) This study reports the immobilization of a new lipase isolated from oleaginous seeds of Pachira aquatica, using beads of calcium alginate (Alg) and poly(vinyl alcohol) (PVA). We evaluated the morphology, number of cycles of reuse, optimum temperature, and temperature stability of both immobilization methods compared to the free enzyme. The immobilized enzymes were more stable than the free enzyme, keeping 60% of the original activity after 4 h at 50°C. The immobilized lipase was reused several times, with activity decreasing to approximately 50% after 5 cycles. Both the free and immobilized enzymes were found to be optimally active between 30 and 40°C. Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristovão Colombo 2265, Jd. Nazaré, CEP 15054000, SP, Brasil Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristovão Colombo 2265, Jd. Nazaré, CEP 15054000, SP, Brasil Department of Pharmacy and Biochemistry, Centro Universitario de Rio Preto, UNIRP, Rua Ivete Gabriel Atique 45, 15025-400 Sao Jos ˜ e do Rio Preto, SP, Brazil