Contributors: Peptides et petits ARN; Laboratoire de Recherche en Sciences Végétales (LRSV); Université Toulouse III - Paul Sabatier (UT3); Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP); Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3); Université Fédérale Toulouse Midi-Pyrénées; Theories and Approaches of Genomic Complexity (TAGC); Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM); Institut de pharmacologie et de biologie structurale (IPBS); Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS); University of Cambridge [UK] (CAM); Centre National de la Recherche Scientifique (CNRS); ANR-10-INBS-0008,ProFI,Infrastructure Française de Protéomique(2010); ANR-16-CE12-0018,biomiPEPs,Vers une compréhension globale des miPEPs(2016); Brun, C.; Infrastructure Française de Protéomique - - ProFI2010 - ANR-10-INBS-0008 - INBS - VALID; Vers une compréhension globale des miPEPs - - biomiPEPs2016 - ANR-16-CE12-0018 - AAPG2016 - VALID; Pichereaux, Carole [0000-0002-7559-5358]; Apollo - University of Cambridge Repository; Deery, Michael [0000-0001-6895-9814]; Russell, Steve [0000-0003-0546-3031]; Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP); Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3); Université de Toulouse (UT); Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
نبذة مختصرة : Recent studies have shown that hundreds of small proteins were occulted when protein coding genes were annotated. These proteins, called alternative proteins, have failed to be annotated notably due to the short length of their open reading frame (less than 100 codons) or the enforced rule establishing that messenger RNAs (mRNAs) are monocystronic. Several alternative proteins were shown to be biologically active molecules and seem to be involved in a wide range of biological functions. However, genome wide exploration of the alternative proteome is still limited to a few species. In the present article we describe a deep peptidomics workflow which enabled the identification of 401 alternative proteins in Drosophila melanogaster. Subcellular localization, protein domains and short linear motifs were predicted for 235 of the alternative proteins identified and point towards specific functions of these small proteins. Several alternative proteins had approximated abundances higher than their canonical counterparts, suggesting that these alternative proteins are actually the main products of their corresponding genes. Finally, we observed fourteen alternative proteins with developmentally regulated expression patterns and ten induced upon heat shock treatment of embryos, demonstrating stage or stress specific production of alternative proteins.
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