Comparison of microbiological culture and polymerase chain reaction in the diagnosis of salmonellosis in Salmonella Typhimurium-infected calves

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:56:33Z No. of bitstreams: 1 S0102-09352011000500029.pdf: 69352 bytes, checksum: 38fe2112a63a62289a122223055bf795 (MD5) Made available in DSpace on 2013-08-22T18:56:33Z (GMT). No. of bitstreams: 1 S0102-09352011000500029.pdf: 69352 bytes, checksum: 38fe2112a63a62289a122223055bf795 (MD5) Previous issue date: 2011-10-01 Made available in DSpace on 2013-09-30T19:50:36Z (GMT). No. of bitstreams: 2 S0102-09352011000500029.pdf: 69352 bytes, checksum: 38fe2112a63a62289a122223055bf795 (MD5) S0102-09352011000500029.pdf.txt: 14832 bytes, checksum: 044f1070326a65f3a07eaf6212b84a14 (MD5) Previous issue date: 2011-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:14:09Z No. of bitstreams: 2 S0102-09352011000500029.pdf: 69352 bytes, checksum: 38fe2112a63a62289a122223055bf795 (MD5) S0102-09352011000500029.pdf.txt: 14832 bytes, checksum: 044f1070326a65f3a07eaf6212b84a14 (MD5) Made available in DSpace on 2014-05-20T13:14:09Z (GMT). No. of bitstreams: 2 S0102-09352011000500029.pdf: 69352 bytes, checksum: 38fe2112a63a62289a122223055bf795 (MD5) S0102-09352011000500029.pdf.txt: 14832 bytes, checksum: 044f1070326a65f3a07eaf6212b84a14 (MD5) Previous issue date: 2011-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The efficiency of microbiological culture and polymerase chain reaction (PCR) for detection of Salmonella Typhimurium is compared in fecal samples of Holstein calves experimentally infected with 10(9) CFU of Salmonella Typhimurium. Seventy-two fecal samples were analyzed by microbiological culture and PCR associated with selenite cystine (SC) and Muller-Kauffmann tethrationate (TMK) selective enrichment broths. Regardless of the selective enrichment broth, the microbiological culture was significantly better than PCR for detection of positive samples of Salmonella Typhimurium. The selective enrichment broths SC and TMK had no effect on the efficiency of the microbiological culture. The SC broth was the best option as selective enrichment associated to PCR. UNESP FCAV UNESP Faculdade de Medicina Veterinária de Araçatuba UNESP FCAV UNESP Faculdade de Medicina Veterinária de Araçatuba