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Characteristics of Raw-Starch Degrading Amylase Bacteria from Natar Hot Spring Lampung

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  • معلومة اضافية
    • بيانات النشر:
      Institute of Research and Community Services Diponegoro University (LPPM UNDIP), 2020.
    • الموضوع:
      2020
    • نبذة مختصرة :
      Indonesia has a diversity of hot spring as a habitat of bacteria. One of the hot springs is Natar hot spring, Lampung. This study is to report the characteristics of a bacterium called Nat1 isolate that produces amylase to degrade raw starch from Natar hot spring. Water samples were taken from hot springs with a temperature of 45 ° C and a pH of 7.0. Nat1 was isolated by screening on the medium of Starch-Luria Bertani at 37 ° C. Its amylase-producing bacteria showed an optimum amylolytic activity of a crude enzyme of Nat1 isolate in soluble starch was 267.2774 U/mL at 60°C. Genotypic identification results using the 16S rRNA gene showed that the Nat1 isolate is identified as Panninobacter phragmatetus . A crude enzyme of Nat1 isolate showed a novel amylase ability and could degrade the raw starch substrates, such as corn and sago, with the amount of reducing sugar for each raw starch, 37.0688 µmol/mg, and 24.2697 µmol/mg . In conclusion, Nat1 amylase is potentially used in industry for its ability to degrade raw starch directly.
    • ISSN:
      2597-9914
      1410-8917
    • الرقم المعرف:
      10.14710/jksa.23.7.238-243
    • Rights:
      OPEN
    • الرقم المعرف:
      edsair.doi.dedup.....9b8a8c39c19484a495b82f27108ebde5