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Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants

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  • معلومة اضافية
    • بيانات النشر:
      Microbiology Society, 2007.
    • الموضوع:
      2007
    • نبذة مختصرة :
      The major outer-membrane proteins RagA and RagB ofPorphyromonas gingivalisare considered to form a receptor complex functionally linked to TonB. In this study,P.gingivalismutants withragA,ragBor both deleted were constructed from strain W83 as the parent to examine the physiological and pathological functions of RagA and RagB. The double-deletion mutant completely lacked both RagA and RagB, whereas the ΔragAmutant reduced RagB expression considerably and the ΔragBmutant produced degraded RagA. Growth of the three mutants in a nutrient-rich medium and synthetic media containing digested protein as a unique nutrient source was similar to that of the parental strain; however, both the ΔragAand ΔragABmutants exhibited very slow growth in a synthetic medium containing undigested, native protein, and the two mutants tended to lose their viability during experiments, although gingipain (protease) activities were unchanged in the mutants. A mouse model showed that the ΔragBmutant had reduced virulence. Cell-surface labelling with biotin and dextran revealed that both RagA and RagB localized on the outermost cell surface. A cross-linking experiment using wild-typeP. gingivalisshowed that RagA and RagB were closely associated with each other. Furthermore, co-immunoprecipitation confirmed that RagA and RagB formed a protein–protein complex. These results suggest that physically associated RagA and RagB may stabilize themselves on the cell surface and function as active transporters of large degradation products of protein and in part as a virulence factor.
    • ISSN:
      1473-5644
      0022-2615
    • الرقم المعرف:
      10.1099/jmm.0.47289-0
    • Rights:
      OPEN
    • الرقم المعرف:
      edsair.doi.dedup.....7728d089654f9b2502e1be4f9c0523f1