Dependence of interleukin-10 quantitative level from genetic polymorphism of toll-like receptors 2 (rs 5743708) in patients with herpes zoster

Onishchenko N. V., Riabokon O. V. Dependence of interleukin-10 quantitative level from genetic polymorphism of toll-like receptors 2 (rs 5743708) in patients with herpes zoster. Journal of Education, Health and Sport. 2019;9(11):66-75. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.3538284 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/27758 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2019; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 25.10.2019. Revised: 04.11.2019. Accepted: 06.11.2019. UDC 616.834-002.152-036.1-076.5:575.174.015.3:616-008.811 Dependence of interleukin-10 quantitative level from genetic polymorphism of toll-like receptors 2 (rs 5743708) in patients with herpes zoster N. V. Onishchenko, O. V. Riabokon Zaporizhzhya State Medical University Department of Infection Diseases Abstract Background. Herpes zoster is urgent problem of the present time, due to increased incidence of severe course with complications in immunocompetent persons, which leads to decrease quality of life and disability. Therefore, the aim of our work was to determine dependence of interleukin-10 concentration on the polymorphism of gene toll-like receptor 2 (rs 5743708) in patients with herpes zoster. Materials and methods. The study included 50 patients with herpes zoster and 40 healthy people. The quantitative content of IL-10 in serum was determined by enzyme-linked immunosorbent assay, determination of single-nucleotide toll-like receptor 2 polymorphism in whole venous blood was performed by real-time polymerase chain reaction. Statistical processing was performed in program "STATISTICA for Windows 13" (StatSoft Inc., No. JPZ804I382130ARCN10-J). Results. In patients with shingles were significantly more likely to detect AA-genotype of gene toll-like receptors 2 (p=0.02). Also AA-genotype was more frequently detected at moderate course of the disease (p = 0.03). It was revealed that in debut of the disease in patients with AA+AG-genotypes, concentration of IL-10 was more highest compared to healthy people and with carriers of GG-genotype (p Conclusions. The AA-genotype of toll-like receptor 2 gene (rs 5743708) is associated with a high chance of reactivation varicella zoster virus and manifestation shingles, moderate course. The allele A of toll-like receptor 2 gene is associated with highest IL-10 concentration, compared with carriers of GG-genotype and healthy people (p˂0,05). Key words: herpes zoster; varicella zoster virus; concentration; interleukin-10; toll-like receptor 2; genetic polymorphism.