نبذة مختصرة : In this study, we evaluated the impact of 5&ndash
50 &mu
M quercetin (QUE) and naringenin (NAR) on extended boar spermatozoa in the BTS (Beltsville Thawing Solution) medium for 72 h. Spermatozoa motion, membrane, acrosome, and DNA integrity were investigated immediately after sample dilution (0 h) as well as after 24 h, 48 h, and 72 h of semen storage. Furthermore, reactive oxygen species (ROS) and superoxide production, as well as the extent of oxidative damage to the sperm proteins and lipids, were assessed to determine the potential of QUE and NAR to prevent a potential loss of sperm vitality due to oxidative stress development. Our results indicate that the most notable parameter influenced by QUE was the mitochondrial activity, which remained significantly higher throughout the experiment (p <
0.001 and p <
0.0001
10 &mu
M), and which correlated with the most prominent maintenance of sperm motility (p <
0.01, 48 h
p <
0.05, 72 h). A significant membrane stabilization (p <
0.01, 24 h and 48 h
0.0001, 72 h) and prevention of lipid peroxidation (p <
0.05, 24 h and 48 h
0.01, 72 h) was primarily observed following administration of 10 and 25 &mu
M NAR
respectively. Administration of 10 &mu
M QUE led to a significant decrease of superoxide (p <
0.0001, 48 h and 72 h) while the most notable decline of ROS generation was recorded in the case of 10 and 25 &mu
M NAR (p <
0.001). This study may provide new information on the specific mechanisms of action involved in the favorable effects of natural biomolecules on spermatozoa.
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