نبذة مختصرة : Research background. Polysaccharides isolated from plants, fungi and bacteria are associated with immunomodulatory effects. Chickpea hull, which is regarded as food industrial waste, contains considerable amounts of antioxidants and bioactive compounds. Experimental approach. In the present study, we investigated the immunomodulatory activity of polysaccharides from kabuli chickpea (Cicer arietinum L.) hull (CHPS). In vitro study was conducted with RAW264.7 cell line while in vivo study was carried out using specific pathogen-free BALB/c mouse animal model. Results and conclusions. In in vitro test with RAW264.7 murine macrophage cells, the three purified fractions of chickpea hull polysaccharides showed potent immunomodulatory activity. Sample CHPS-3 showed stronger effect on cell viability, promoted the phagocytosis index to a greater extent and had the best effect on acid phosphatase activity. Moreover, it was found that CHPS-3 significantly (p
Pozadina istraživanja. Polisaharidi izolirani iz biljaka, gljivica i bakterija imaju dokazani imunomodulacijski učinak. Ljuska slanutka, koja se smatra otpadom prehrambene industrije, bogata je antioksidansima i bioaktivnim spojevima. Eksperimentalni pristup. U ovom smo radu ispitali imunomodulacijsku aktivnost polisaharida iz ljuske slanutka (Cicer arietinum L.) vrste kabuli. Ispitivanje je provedeno in vitro na staničnoj liniji RAW264,7, a in vivo na BALB/c miševima, uzgojenim u uvjetima bez patogena specifičnih za miševe. Rezultati i zaključci. U ispitivanju in vitro na makrofagu sličnim stanicama RAW264,7, tri pročišćene frakcije polisaharida iz ljuske slanutka imale su izraženu imunomodulacijsku aktivnost. Uzorak iz treće frakcije imao je veći utjecaj na preživljavanje stanica, znatnije je poboljšao fagocitni indeks i u najvećoj mjeri utjecao na aktivnost kisele fosfataze. Osim toga, taj je uzorak bitno (p
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