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Stimulation of human olfactory receptor 17-40 with odorants probed by surface plasmon resonance

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  • معلومة اضافية
    • Contributors:
      Ampère; École Centrale de Lyon (ECL); Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon); Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS); V.E. Lashkaryov Institute of Semiconductor Physics; National Academy of Sciences of Ukraine (NASU); Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA); Institut National de la Recherche Agronomique (INRA); Institute of Molecular Biology and Genetics of NAS of Ukraine; Sciences Analytiques (SA); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS); Vladimir I. Chegel appreciates Wnancial support from NATO CLG grant PDD (CP)-(CBP.NUKR.CLG981776). Irina Benilova was supported by EGIDE (France) through the doctoral Bourse EiVel.; Ampère (AMPERE); Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
    • بيانات النشر:
      Springer Science and Business Media LLC, 2008.
    • الموضوع:
      2008
    • نبذة مختصرة :
      International audience; We report here the results of human olfactory receptor (OR) 17-40 stimulation with some odorants probed by means of the double-channel surface plasmon resonance platform NanoSPR-6. OR 17-40 tagged with N-terminal cmyc sequence was heterologously co-expressed with Gαolf protein in yeast, and receptor-carrying nanosomes were prepared from yeast membrane fraction. Then, receptors were specifically captured via anti-cmyc antibody attached to the gold-coated substrate in orientated or random way. Measurement of odorants effects were carried out in the presence of GTP-γ-S in differential mode in order to compensate bulk changes of refractive index. For the first time, biosensing efficiency of olfactory films was discussed in terms of their thickness and Gαolf accessibility to GTP-γ-S. Bell-shaped response profile with two maxima (near 1 nM and near 1 μM) was established for helional, which is documented as highly specific agonist of OR 17-40. Unrelated odorant heptanal used as control, did not evoke significant variations of differential signal.
    • ISSN:
      1432-1017
      0175-7571
    • الرقم المعرف:
      10.1007/s00249-008-0272-5
    • الرقم المعرف:
      10.1007/s00249-008-0272-5⟩
    • Rights:
      CLOSED
    • الرقم المعرف:
      edsair.doi.dedup.....21d81f75dab2c24b82fa31320e052190