Vitamín D v úlohe reparácie DNA senescentných buniek poškodených peroxidom vodíka

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SÚHRN V rámci našich experimentov sme sa zamerali na sledovanie protektívnych účinkov vitamínu D na senescentné (starnúce) bunky MRC-5 v pasáži 21 (p21) a nesenescentné (mladé) bunky v pasáži 8 (p8) pred a po poškodení peroxidom vodíka. MTT testom sme zistili, že vitamín D zvýšil prežívanie buniek p8 nad 100 % v koncentrácii 20 µmol/L a 25 µmol/L. V prípade buniek p21 vitamín D zvýšil prežívanie buniek nad 100 % v koncentrácii 10 µmol/L a 25 µmol/L. Tieto zvýšenia však neboli signifikantné. Následne sme sledovali vplyv vitamínu D na poškodenie DNA buniek indukované peroxidom vodíka a zistili sme, že vitamín D vykazuje nielen protektívny, ale aj reparačný účinok. Tento účinok bol signifikantný v koncentráciách 1, 10, 20, 25 a 50 µmol/L. ABSTRACT In our experiments we focused on monitoring the protective effects of vitamin D on senescent MRC-5 cells at passage 21 (p21) and non-senescent cells at passage 8 (p8) before and after hydrogen peroxide damage. By MTT test we found that vitamin D increased the survival of p8 cells above 100 % at a concentration of 20 µmol/L and 25 µmol/L. In the case of p21 cells, vitamin D increased cell survival above 100 % at a concentration of 10 µmol/L and 25 µmol/L. However, these increases were not significant. Subsequently, we monitored the effect of vitamin D on hydrogen peroxide-induced DNA damage in cells and found that vitamin D has not only a protective but also a repair effect. This effect was significant at concentrations of 1, 10, 20, 25 and 50 µmol/L.
Táto práca bola finančne podporená - grantom EU z programu CBC, Interreg V-A-NutriAging V-0014 - a grantom Vedeckej grantovej agentúry MŠVVaŠ SR a SAV (VEGA) 1/0314/19. The study was supported by the following grants: - NutriAging project realized under the Interreg V-A Cross-border Cooperation Program V-A-NutriAging V-0014 - The Ministry of Education, Science, Research and Sport of the Slovak Republic and the Slovak Academy of Sciences (VEGA) 1/0314/19.