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Genomic and transcriptomic variation defines the chromosome-scale assembly of Haemonchus contortus, a model gastrointestinal worm

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  • معلومة اضافية
    • Contributors:
      The Wellcome Trust Sanger Institute [Cambridge]; Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences; University of Glasgow; Moredun Research Institute [Penicuik, UK] (MRI); Institute of Parasitology [Ste Anne de Bellevue, Québec]; McGill University = Université McGill [Montréal, Canada]; Royal (Dick) School of Veterinary Studies; European Molecular Biology Laboratory [Hinxton]; Faculty of Veterinary Medicine, University of Calgary; University of Calgary; Infectiologie et Santé Publique (UMR ISP); Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); University of Veterinary and Animal Sciences [Lahore, Pakistan] (UVAS); Wellcome's core funding of the Wellcome Sanger Institute (grant WT206194), Wellcome Trust Project Grant to JSG (grant 067811), the Biotechnology and Biological Sciences Research Council (BBSRC) grants (BB/M003949/1, BB/P024610/1 and BB/K020048/1); Sallé, Guillaume; College of Medical, Veterinary and Life Sciences [Glasgow]; Infectiologie et Santé Publique (ISP); The Wellcome Trust Sanger Institute Cambridge; Moredun Research Institute Penicuik, UK (MRI); Institute of Parasitology Ste Anne de Bellevue, Québec; McGill University = Université McGill Montréal, Canada; European Molecular Biology Laboratory Hinxton; University of Veterinary and Animal Sciences Lahore, Pakistan (UVAS)
    • بيانات النشر:
      Springer Science and Business Media LLC, 2020.
    • الموضوع:
      2020
    • نبذة مختصرة :
      Haemonchus contortus is a globally distributed and economically important gastrointestinal pathogen of small ruminants and has become a key nematode model for studying anthelmintic resistance and other parasite-specific traits among a wider group of parasites including major human pathogens. Here, we report using PacBio long-read and OpGen and 10X Genomics long-molecule methods to generate a highly contiguous 283.4 Mbp chromosome-scale genome assembly including a resolved sex chromosome for the MHco3(ISE).N1 isolate. We show a remarkable pattern of conservation of chromosome content with Caenorhabditis elegans, but almost no conservation of gene order. Short and long-read transcriptome sequencing allowed us to define coordinated transcriptional regulation throughout the parasite’s life cycle and refine our understanding of cis- and trans-splicing. Finally, we provide a comprehensive picture of chromosome-wide genetic diversity both within a single isolate and globally. These data provide a high-quality comparison for understanding the evolution and genomics of Caenorhabditis and other nematodes and extend the experimental tractability of this model parasitic nematode in understanding helminth biology, drug discovery and vaccine development, as well as important adaptive traits such as drug resistance.
    • File Description:
      application/pdf
    • ISSN:
      2399-3642
    • الرقم المعرف:
      10.1038/s42003-020-01377-3
    • الرقم المعرف:
      10.60692/fw4cg-mn512
    • الرقم المعرف:
      10.60692/p11ft-qq684
    • Rights:
      CC BY
    • الرقم المعرف:
      edsair.doi.dedup.....204220c63dff3a92fbe0b1e10f542dc3