Association between endothelial nitric oxide synthase and the renin-angiotensin-aldosterone system polymorphisms, blood pressure and training status in normotensive/pre-hypertension and hypertensive older adults: a pilot study

Made available in DSpace on 2022-04-29T08:45:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Introduction:Variations in blood pressure (BP) are, in part, genetically determined and some polymorphisms of renin-angiotensin- aldosterone system (RAAS) and synthase of endothelial nitric oxide (eNOS) have been related to hypertension (HT). Conversely, physical exercise is considered a non-pharmacological tool for HT control, treatment, and prevention. Objective: The purpose of this study is to investigate the relationship between eNOS and RAAS polymorphisms, their epistatic interaction, and the respective humoral factors in the BP control in normotensive/pre-hypertension and hypertensive older adults and how this relationship can be modulated by training status (TS) level. Methods:A total of 155 older adults (66.94 ± 6.83 years old) performed the following evaluations: AAHPERD battery test to determine the general functional fitness index (GFFI), systolic and diastolic blood pressure (SBP and DBP), blood collection for DNA extraction, analysis of eNOS gene polymorphisms rs2070744; rs61722009 and rs1799983 and RAAS polymorphisms rs699; rs1799752 and rs5186, and quantification of ACE activity (Fluorimetric Assay) and nitrite concentration (Chemiluminescence Method). Results and Conclusion:Good TS level appears to exert greater influence on SBP for G2 and G3 (G1: 125.79 ± 14.03/ G2: 119.91 ± 11.72/G3: 119.71 ± 10.85) and on NO2 for G3 (G1: 0.42 ± 0.25/ G2: 0.54 ± 0.45/ G3: 0.71 ± 0.52). No associations were observed between eNOS and RAAS polymorphisms, but the epistasis was identified between eNOS polymorphism, rs2070744, and RAAS polymorphism, rs699, revealing a statistically significant interaction (p = .0235) with training score of 0.63, a training test accuracy of 0.61 and a cross-validation consistency of 10/10. This result suggests an increased risk of hypertension. Department of Physical Education Post-Graduate Program in Movement Sciences School of Sciences Sao Paulo State University (Unesp) Department of Psychiatric Nursing and Human Sciences University of São Paulo (USP) Department of Genetics Faculty of Medicine of Ribeirão Preto University of São Paulo (USP) Department of Pharmacology Faculty of Medicine of Ribeirão University of São Paulo (USP) Institute of Biological Sciences Department of Genetics Ecology and Evolution University of Minas Gerais Federal Department of Pharmacology Bauru School of Dentistry University of São Paulo (USP) Department of Physical Education Center for Noncommunicable Diseases Aging and Exercise Studies (CEDEE) School of Sciences Sao Paulo State University (Unesp) Department of Physical Education Post-Graduate Program in Movement Sciences School of Sciences Sao Paulo State University (Unesp) Department of Physical Education Center for Noncommunicable Diseases Aging and Exercise Studies (CEDEE) School of Sciences Sao Paulo State University (Unesp)