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Three steps towards comparability and standardization among molecular methods for characterizing insect communities

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  • معلومة اضافية
    • بيانات النشر:
      The Royal Society, 2024.
    • الموضوع:
      2024
    • نبذة مختصرة :
      Molecular methods are currently some of the best-suited technologies for implementation in insect monitoring. However, the field is developing rapidly and lacks agreement on methodology or community standards. To apply DNA-based methods in large-scale monitoring, and to gain insight across commensurate data, we need easy-to-implement standards that improve data comparability. Here, we provide three recommendations for how to improve and harmonize efforts in biodiversity assessment and monitoring via metabarcoding: (i) we should adopt the use of synthetic spike-ins, which will act as positive controls and internal standards; (ii) we should consider using several markers through a multiplex polymerase chain reaction (PCR) approach; and (iii) we should commit to the publication and transparency of all protocol-associated metadata in a standardized fashion. For (i), we provide a ready-to-use recipe for synthetic cytochromecoxidase spike-ins, which enable between-sample comparisons. For (ii), we propose two gene regions for the implementation of multiplex PCR approaches, thereby achieving a more comprehensive community description. For (iii), we offer guidelines for transparent and unified reporting of field, wet-laboratory and dry-laboratory procedures, as a key to making comparisons between studies. Together, we feel that these three advances will result in joint quality and calibration standards rather than the current laboratory-specific proof of concepts.This article is part of the theme issue ‘Towards a toolkit for global insect biodiversity monitoring’.
    • File Description:
      application/pdf
    • ISSN:
      1471-2970
      0962-8436
    • الرقم المعرف:
      10.1098/rstb.2023.0118
    • Rights:
      CC BY
      URL: http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
    • الرقم المعرف:
      edsair.doi.dedup.....03ff7e64b3a9c0d89b83f67d0946c297