Construction of a lysosome-targetable ratiometric fluorescent probe for H2O2tracing and imaging in living cells and an inflamed model

Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS) with unique destructive oxidation properties, can be produced in lysosomes to fight off pathogens. Although many fluorescent probes have been developed for the detection and imaging of H2O2, the development of a ratiometric fluorescent probe for H2O2 detection and imaging in lysosomes and an inflammation model remains rather scarce. Therefore, it is important to develop an efficient tool for monitoring H2O2 in inflamed tissues to evaluate the physiological and pathological relationship between inflammation and lysosomal H2O2. In this work, a new naphthalimide-based lysosome-targeting fluorescent probe (NPT-H2O2) for ratiometric detection and imaging was developed in vitro and in vivo. The probe exhibited two well-resolved emission peaks separated by 125 nm, rapid response (