Dentin surface and hybrid layer morphological analysis after use of a copaiba oil- based dentin biomodifier

This study evaluated dentin surface and hybrid layer’s morphology, using copaíba (CO) emulsion as dentin biomodifier before traditional and self-etching adhesive, by environmental scanning electron microscopy (ESEM). Ninety-six third sound molars were divided into 16 groups, according to the dentin substrate, tested substances, and the adhesive system used. The conditioned dentin surface treated with clorexidina (CHX) 2% showed phosphate salts on two dentin substrates as well as the sound conditioned or not surface treated with calcium hydroxide solution (CHS), where the mineral precipitate deposition obstructs the tubules lumen. A significant difference (p