Factors Affecting The Indirect Immunoperoxidase Staining Of Infectious Bursal Disease Virus Antigens In Cells

THE application of the Immunoperoxidase (IP) antibody technique for the definitive diagnosis of Infectious Bursal Disease (IBD) in chickens has earlier been described (Okeke and Lang, 1982). During that study it was noted that the Indirect method of IP was more sensitive in the demonstration of IBD viral antigens than the direct method. Consequently, more formation was sought on the specific technical parameter and the consequences that resulted by deviations from the codified procedures employed in the Indirect technique with the aim of obtaining an even better result. Studies of pH, serum dilutions, serum incubation time, conjugate dilutions, conjugate incubation time and developing time were conducted to find out what Influence such factors could have on the final staining product.