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An Undergraduate Laboratory Experiment with Real-World Applications: Utilizing Templateless Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction to Test for SARS-CoV-2 RNA
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- المؤلفون: Crane, Julia; Page, Shallee T. (ORCID Page, Shallee T. (ORCID 0000-0002-3557-4122)
- اللغة:
English
- المصدر:
Biochemistry and Molecular Biology Education. Jan-Feb 2022 50(1):142-148.
- الموضوع:
2022
- نوع التسجيلة:
Journal Articles
Reports - Research
- معلومة اضافية
- Peer Reviewed:
Y
- المصدر:
7
- Sponsoring Agency:
National Institute of General Medical Sciences (NIGMS) (DHHS/NIH)
- Contract Number:
P20GM103506
- Education Level:
Higher Education
Postsecondary Education
- الموضوع:
- الرقم المعرف:
10.1002/bmb.21593
- ISSN:
1470-8175
- نبذة مختصرة :
We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published protocols, we assembled a successful project that illustrated a topical exercise similar to real-world assay development. The exercise is aimed at upper-level undergraduates and requires 3 weeks of laboratory periods. The students were able to design and test experimental protocols, while learning about RNA detection. This project could be utilized in upper-level classes including molecular biology, biochemistry, biotechnology, or for independent research projects.
- نبذة مختصرة :
As Provided
- الموضوع:
2022
- الرقم المعرف:
EJ1326383
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