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An Undergraduate Laboratory Experiment with Real-World Applications: Utilizing Templateless Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction to Test for SARS-CoV-2 RNA

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اقرأ على الانترنت اقرأ أكثر حفظ في قائمتي
  • المؤلفون: Crane, Julia; Page, Shallee T. (ORCID Page, Shallee T. (ORCID 0000-0002-3557-4122)
  • اللغة:
    English
  • المصدر:
    Biochemistry and Molecular Biology Education. Jan-Feb 2022 50(1):142-148.
  • الموضوع:
    2022
  • نوع التسجيلة:
    Journal Articles
    Reports - Research
  • معلومة اضافية
    • Peer Reviewed:
      Y
    • المصدر:
      7
    • Sponsoring Agency:
      National Institute of General Medical Sciences (NIGMS) (DHHS/NIH)
    • Contract Number:
      P20GM103506
    • Education Level:
      Higher Education
      Postsecondary Education
    • الموضوع:
      Undergraduate Students; College Science; Science Instruction; Scientific Research; Laboratory Experiments; Genetics; COVID-19; Microbiology; Molecular Biology; Biochemistry; Biotechnology
    • الرقم المعرف:
      10.1002/bmb.21593
    • ISSN:
      1470-8175
    • نبذة مختصرة :
      We present a course-embedded undergraduate research module that involves real-time polymerase chain reaction testing for the presence of SARS-CoV-2 in environmental samples. A positive control RNA was constructed and two RNA extraction methods were compared and a range of primers were available to compare. Using a combination of published protocols, we assembled a successful project that illustrated a topical exercise similar to real-world assay development. The exercise is aimed at upper-level undergraduates and requires 3 weeks of laboratory periods. The students were able to design and test experimental protocols, while learning about RNA detection. This project could be utilized in upper-level classes including molecular biology, biochemistry, biotechnology, or for independent research projects.
    • نبذة مختصرة :
      As Provided
    • الموضوع:
      2022
    • الرقم المعرف:
      EJ1326383