New positive/negative selectable markers for mammalian cells on the basis of Blasticidin deaminase-thymidine kinase fusions.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Print ISSN: 0305-1048 (Print) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.

Genetic Markers*; Aminohydrolases/*genetics ; Anti-Bacterial Agents/*pharmacology ; Thymidine Kinase/*genetics; 3T3 Cells ; Animals ; Aspergillus/enzymology ; Bacillus cereus/enzymology ; Drug Resistance ; Drug Resistance, Microbial ; Escherichia coli/drug effects ; Ganciclovir/pharmacology ; Genetic Vectors/genetics ; Mice ; Nucleosides/pharmacology ; Recombinant Fusion Proteins ; Selection, Genetic ; Simplexvirus/enzymology

Two positive and negative selectable markers were created for use in mammalian cells. They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by their ability to induce BlaS resistance and negatively on the induced sensitivity towards gancyclovir (GANC). Both constructs are also expressed in Escherichia coli and transfer BlaS resistance to this organism as well, making these markers very suitable for the construction of shuttle vectors.

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0 (Anti-Bacterial Agents)
0 (Genetic Markers)
0 (Nucleosides)
0 (Recombinant Fusion Proteins)
83U64J9U23 (blasticidin S)
EC 2.7.1.21 (Thymidine Kinase)
EC 3.5.4.- (Aminohydrolases)
EC 3.5.4.23 (blasticidin S deaminase)
P9G3CKZ4P5 (Ganciclovir)

Date Created: 19980610 Date Completed: 19980702 Latest Revision: 20190501

20231215

PMC147567

10.1093/nar/26.10.2508

9580709