Meiotic double-strand breaks in yeast artificial chromosomes containing human DNA.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Print ISSN: 0305-1048 (Print) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.

Chromosomes, Artificial, Yeast/*genetics ; DNA/*genetics ; DNA Damage/*genetics ; Meiosis/*genetics; Chromatin ; Deoxyribonuclease I ; Enzyme Inhibitors/pharmacology ; Globins/genetics ; Humans ; Recombination, Genetic ; Saccharomyces cerevisiae/genetics ; Single-Strand Specific DNA and RNA Endonucleases ; Teniposide/pharmacology ; Topoisomerase II Inhibitors

Meiotic recombination in the yeast Saccharomyces cerevisiae is initiated by double-strand breaks (DSB) in chromosomal DNA. These DSB, which can be mapped in the rad 50S mutant yeast strain, are caused by a topoisomerase II-like enzyme, the protein Spo11. Evidence suggests that this protein is located in the axial element of the meiotic chromosome which implies that the DSB are located in these chromosomes in the vicinity of the bases of the DNA loops. We have found that in the yeast artificial chromosomes carrying human DNA, at the level of resolution obtained by pulsed field gel electrophoresis (PFGE), the meiotic DSB in the diploid yeast are co-localized with the DNase I hypersensitive sites (HS) in a haploid strain of yeast. These HS are located close to sequences which, under stress, have the potential to form secondary structures containing unpaired nucleotides. Clusters of such sequences could be a hallmark of the bases of the chromatin loops.

Nat Genet. 1996 Aug;13(4):481-4. (PMID: 8696347)
Mol Gen Genet. 1998 Feb;257(3):292-8. (PMID: 9520263)
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6384-8. (PMID: 3879975)
Cell. 1986 Jan 31;44(2):273-82. (PMID: 3002631)
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5194-8. (PMID: 2425363)
Annu Rev Biochem. 1988;57:159-97. (PMID: 3052270)
Bioessays. 1988 Nov;9(5):151-3. (PMID: 3071365)
EMBO J. 1990 Feb;9(2):567-71. (PMID: 2303042)
EMBO J. 1990 Apr;9(4):1319-27. (PMID: 2323342)
Mol Cell Biol. 1990 Aug;10(8):4324-33. (PMID: 2370867)
Trends Biochem Sci. 1990 Jun;15(6):222-7. (PMID: 2166364)
Genomics. 1991 Aug;10(4):976-84. (PMID: 1916829)
Science. 1992 Jan 10;255(5041):195-7. (PMID: 1553545)
Nature. 1992 May 28;357(6376):282-3. (PMID: 1589033)
Biochimie. 1992 Nov;74(11):1019-23. (PMID: 1335757)
Biochemistry. 1994 Jan 11;33(1):367-74. (PMID: 8286358)
Science. 1994 Jan 28;263(5146):515-8. (PMID: 8290959)
Proc Natl Acad Sci U S A. 1994 May 10;91(10):4422-6. (PMID: 8183924)
Cell. 1994 Jul 1;77(7):977-91. (PMID: 8020104)
Curr Opin Genet Dev. 1994 Apr;4(2):260-4. (PMID: 8032204)
Genomics. 1994 Jul 1;22(1):108-17. (PMID: 7959756)
EMBO J. 1994 Dec 1;13(23):5754-63. (PMID: 7988571)
Chromosoma. 1994 Oct;103(6):401-7. (PMID: 7859560)
J Biomol Struct Dyn. 1994 Dec;12(3):605-23. (PMID: 7727061)
J Biol Chem. 1995 Aug 4;270(31):18685-90. (PMID: 7629201)
Biochem Biophys Res Commun. 1995 Aug 4;213(1):147-53. (PMID: 7639728)
Chromosoma. 1995 Nov;104(3):183-8. (PMID: 8529458)
Int Rev Cytol. 1995;162A:279-388. (PMID: 8575883)
Mol Cell Biol. 1996 May;16(5):2037-43. (PMID: 8628269)
Biochemistry. 1996 Feb 20;35(7):2239-52. (PMID: 8652565)
Annu Rev Cell Dev Biol. 1996;12:305-33. (PMID: 8970729)
Cell. 1997 Feb 7;88(3):375-84. (PMID: 9039264)
Nature. 1997 Mar 27;386(6623):414-7. (PMID: 9121560)
Cell. 1997 Aug 22;90(4):625-34. (PMID: 9288743)
Cell. 1984 Nov;39(1):223-32. (PMID: 6091913)

0 (Chromatin)
0 (Enzyme Inhibitors)
0 (Topoisomerase II Inhibitors)
9004-22-2 (Globins)
9007-49-2 (DNA)
957E6438QA (Teniposide)
EC 3.1.21.1 (Deoxyribonuclease I)
EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases)

Date Created: 19980610 Date Completed: 19980702 Latest Revision: 20190501

20221213

PMC147548

10.1093/nar/26.10.2415

9580694